Fig. 1

Identification of high glucose-regulated SETD7 in diabetic vessels. A The protein expression of SETD7 in aortic tissues from STZ-induced mice (n = 5) and db/db mice (n = 5). B Representative images of wound healing assay photographed at 0, 12, and 24 h of culture (100 ×). C Representative images of the Transwell assay photographed after 36 h of culture (200 ×). D The mRNA level of Setd7 in high glucose-induced RAECs (33 mM D-glucose for 24 h). E The protein expression of SETD7 in RAECs treated with or without high glucose concentrations (11 mM, 22 mM) for 72 h. F The protein expression of SETD7 in RAECs treated with or without 22 mM high glucose for 36–72 h. G Representative images of immunofluorescence staining of SETD7 and COX-2 in RAECs treated with or without high glucose. Scale bars, 50 μm. Statistical significances were calculated using (A–D) two tail student’s t test, (E, F) one-way ANOVA, Tukey’s multiple comparisons tests. Data are expressed as the mean ± SEM, statistical analysis revealed a significant difference with *p < 0.05, **p < 0.01, and ***p < 0.001