Fig. 5

Adipose derived EVs and miRNA regulates glucose metabolism and fetal growth. (Right) Overview of experimental design. Pregnant mice (n = 4 per group) received daily doses of 8 µg/kg of EVs from women with normal pregnancy or GDM, EVs-transfected with miR-515-5p or PBS as Control. (A) Maternal blood glucose and (B) the area under the blood glucose response curve were measured via tail bleeding on GD17.5 after 4 days of EVs or PBS injection. (C) Mice weight, (D) litter size (number of fetuses per pregnant mouse), (E) Fetal weight and (F) placental weight was measured on post-operative after 5 days of EVs injection. (G) Fetal glucose was measured using fetal blood on PO post during fetal and tissue collection. P values in the figures represent the results of a one-way ANOVA, followed by a Bonferroni post hoc test. Data displayed as mean ± SEM. *p < 0.05 **p < 0.005, ***p < 0.0005,****p < 0.001. Fetal weight, placental weight and fetal glucose are presented as the average fetal weight per dam, adjusted for litter size, and compared these averages across treatment groups