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Fig. 3 | Cardiovascular Diabetology

Fig. 3

From: Extracellular vesicle-associated miR-515-5p from adipose tissue regulates placental metabolism and fetal growth in gestational diabetes mellitus

Fig. 3

Effects of adipose tissue-derived EVs associated miRNA on placental cells. (A) Schematic diagram of miRNA transfection in primary trophoblast experimental design. Effect of adipose tissue-derived EVs associated miRNA, (B) glucose uptake, and (C) fatty acid uptake by 2-NBDG and BODIPY staining (n = 6 patients). Glucose and fatty acid accumulation measured as relative fluorescence unit (rfu). The TNF- induced release of (D) CXCL8, (E) IL-6, (F) CXCL1, (G) CCL2, and (H) CCL5 measured using ELISA (n = 6 patients). All assays were performed after 48 h of transfection with miRControl and miRNA mimics. Repeated measure one-way ANOVA was performed with Bonferroni test. Data presented as mean ± SEM. *p < 0.05 **p < 0.005

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Cardiovascular Diabetology

ISSN: 1475-2840