Table 3 Corresponding Antibody list for western blot experiment
Antibody | Product NO | Company | Dilution |
|---|---|---|---|
TNF-α | AMC3012 | Invitrogen | 1:500 |
IL-6 | Sc.32296 | Santa Cruz Biotechnology | 1:1000 |
IL-6 Receptor | MA5-29721 | Invitrogen | 1:1000 |
eNOS | 32027 | Cell Signaling Technology | 1:1000 |
HSP-27 | ab2790 | Abcam | 1:1000 |
HSP-70 | ab2787 | Abcam | 1:1000 |
PKG | 13511 | Cell Signaling Technology | 1:1000 |
p38 MAPK | 9212S | Cell Signaling Technology | 1:1000 |
NF-κB p65(D14E12) | 8242 | Cell Signaling Technology | 1:1000 |
ERK (1/2) p44/42 MAPK | 4695 | Cell Signaling Technology | 1:1000 |
AKT pan (C67E7) | 4691 | Cell Signaling Technology | 1:1000 |
Phospho-AKT (S473) (736E11) | 3787 | Cell Signaling Technology | 1:1000 |
AMPK (D5A2) | 5831 | Cell Signaling Technology | 1:1000 |
Phospho-AMPK (Thr172) (40H9) | 2535 | Cell Signaling Technology | 1:1000 |
mTOR (7C10) | 2983 | Cell Signaling Technology | 1:1000 |
Phospho-mTOR (Ser2448) | 2971 | Cell Signaling Technology | 1:1000 |
NLRP3 (D4D8T) | 15101 | Cell Signaling Technology | 1:1000 |
PI3 Kinase p110α (C73F8) | 4249 | Cell Signaling Technology | 1:1000 |
GSK-3β | MA5-1509 | Thermo Fisher Scientific | 1:1000 |
GAPDH-anti mouse | 97166 | Cell Signaling Technology | 1:10000 |
GAPDH-anti rabbit | 2118 | Cell Signaling Technology | 1:10000 |