Fig. 4
Oxidative Stress Markers in Inflammation in Left Ventricular Biopsies from Patients with HFpEF, with (+ DM) and without Concomitant Diabetes (-DM). A 3-Nitrotyrosine levels. B Ratio of myocardial oxidized glutathione (GSSG) over reduced glutathione (GSH). C Myocardial hydrogen peroxide (H2O2) levels. D Myocardial lipid oxidation (LPO) levels. Data are represented as box and whisker plots (median, 25th to 75th percentiles, minimum, and maximum (n = 10 samples/group). P-values are derived from an unpaired t-test; * P < 0.05, ** P < 0.01. E Fpassive at sarcomere length 1.8–2.4 µm in the presence or absence of Mito-TEMPO. Curves are second-order polynomial fits to the means (± SEM; n = 30–36/5 cardiomyocytes/heart per group), * P < 0.05 HFpEF -DM vs. HFpEF + DM, ‡P < 0.05 HFpEF -DM vs. HFpEF -DM after Mito-TEMPO, † P < 0.05 HFpEF + DM vs. HFpEF + DM after Mito-TEMPO treatment by one-way ANOVA. P-values were corrected for multiple comparisons by the Tukey method