Fig. 6

BDH1 inhibits LCN2-mediated NF-κB activation. A Immunoprecipitation and western blot analysis showing the interaction between LCN2 and NF-κB. B Immunoprecipitation and western blot analysis showing the interaction between LCN2 and NF-κB in Vector- or Flag-BDH1-transfected H9C2 cells with or without PA treatment. C, D Immunoprecipitation and western blot analysis showing the interaction between RPS3 and NF-κB and the phosphorylation level of NF-κB in H9C2 cells transfected with Vector or Flag-LCN2 (C) and siNC or siLcn2 (D). E, F Immunostaing analysis showing the nuclear localization of NF-κB in Vehicle- or PA-treated H9C2 cells transfected with Vector, Flag-BDH1 or Flag-BDH1 accompanied with Flag-LCN2 (E) and PA-treated H9C2 cells transfected with siNC, siBdh1 or siBdh1 accompanied with siLcn2 (F). G Immunostaining analysis showing the nuclear localization of NF-κB in the cardiac tissues from db/m or db/db mice which were injected with AAV9-Con, AAV9-Bdh1 or AAV9-Bdh1 accompanied with AAV9-Lcn2. Bars: 100 μm in the upper panels and 10 μm in the amplified panels. H Illustration showing that BDH1 inhibits LCN2-mediated NF-κB activation. All results are representative of three independent experiments