Fig. 1

Study design overview: timeline and methodology. Wild Type C57BL6/J male and female mice were fed normal (ND) or a western diet (WD) for 150 days (n = 10 mice in each group). Transthoracic echocardiography was performed on all mice at 455, 530, and 750/640 days. Bulk mRNA sequencing was performed on left ventricular cardiac tissue samples collected at 530 and 750 days for male mice (n = 5 mice in each group), and 530 and 640 days for female mice (n = 5 mice in each group)