Fig. 2

LncRNA signature in LV tissue of db + or db/db hearts with or without MI. A Schematic outline of experimental design and downstream bioinformatic analysis. B Distribution of transformed RNA sequencing data. C Principal component analysis of RNA sequencing data. D A heat map of lncRNAs differentially expressed at 3 days following left anterior descending coronary artery (LAD) artery ligation or sham surgery in db + or db/db mice showing distinct profiles. E Volcano plots showing Log₂-fold changes for the differentially regulated lncRNA in the LV tissue of db + or db/db hearts at 3 days post sham or MI surgery. Red dots indicate significantly up-regulated lncRNAs; blue dots indicate down-regulated lncRNAs. F A table showing top five up or down-regulated lncRNAs in the LV tissue of db + or db/db hearts at 3 days post sham or MI surgery. G–H Normalized counts of the top 5 up or downregulated lncRNA in the LV tissue of db + or db/db hearts at 3 days post sham or MI surgery. Data are shown as Log₂ fold change > 2, p < 0.001, and FDR < 0.05