Fig. 7

The transcription factor STAT1 positively regulates STING expression. A Heatmap of aging-associated genes identified in hearts from Ctrl, HFpEF and EMPA-treated mice by proteomics analysis; B, C Immunoblot analysis and quantification of Bax protein in heart tissues, n = 3; D–G Quantification and immunoblot analysis of cGAS, STING and IL6 protein in heart tissues, n = 3; H Representative immunoblot images of Bax and STING in H9C2 cells exposed to IFN-γ at different concentrations; I Representative immunoblot images of STAT1, STING and Bax protein in H9C2 cells treated with IFN-γ after transfection with STAT1 siRNA; J Representative immunoblot images of STAT1, STING, p53 and p21 protein in STAT1-knockdown (KD) cells; K, L mRNA levels of STING and p21 in control and STAT1-KD cells, n = 3; M Representative immunoblot images of STAT1, cGAS, STING and IL6 protein in STAT1-KD cells treated with IFN-γ; N Representative immunoblot images of STING and IL6 protein in H9C2 cells treated with IFN-γ and EMPA pretreatment. All the data are shown as the means ± SEM; one-way ANOVA with Tukey’s multiple comparisons test or unpaired Student’s t-test was used for comparisons; *P < 0.05, **P < 0.01