Fig. 5

Stat1 activation downstream of IFN-γ mediated cardiomyocyte senescence. A The serum levels of IFN-γ in different groups, n = 4–8; B Representative images of SA-β-gal staining in H9C2 cells exposed to IFN-γ at different concentrations for 72 h or normal culture after 200 µM H₂O₂ treatment for 2 h, scale bars: 100 μm; C Quantification of SA-β-gal-positive cells in different groups, n = 4; D Representative immunoblot images of p-STAT1, t-STAT1 and γ-H2AX in H9C2 cells exposed to IFN-γ at different concentrations; E Immunoblot analysis for STAT1 and γ-H2AX in H9C2 cells after STAT1 inhibition; F, G Quantification and representative images of SA-β-gal staining in H9C2 cells exposed to 50 µM IFN-γ after transfection with STAT1 siRNA, n = 3; scale bars:100 μm. All the data are shown as the means ± SEM; one-way ANOVA with Tukey’s multiple comparisons test was used for comparisons; *P < 0.05, **P < 0.01