Fig. 4

EMPA inhibits the cardiac inflammatory response and aging in HFpEF mice. A The serum levels of LDH activity in different groups, n = 6; B, C The levels of MDA content (n = 6) and SOD activity (n = 3) in heart tissues; D, E Immunoblot analysis and quantification of SOD2 protein, n = 4; F The mRNA expression of Stat1, Ifit1, Ifit3, Ifi35 and Ifi47 in the heart, n = 6–8; G, H Representative western blot image and quantification of Stat1, Ifit1 and Ifit3 in the heart, n = 3; I, J Representative immunoblot images and quantification of p16 in the heart, n = 3; K, L Immunoblot analysis and relative expression of p21 in the heart, n = 4; M Representative images of immunohistochemical staining of p21, scale bars:20 μm. All the data are shown as the means ± SEM; one-way ANOVA with Tukey’s multiple comparisons test was used for comparisons; *P < 0.05, **P < 0.01